癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
ISCU Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
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Application 
| WB, E |
|---|---|
| Primary Accession | Q9H1K1 |
| Other Accession | NP_055116.1 |
| Reactivity | Human, Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 17999 Da |
| Antigen Region | 138-167 aa |
| Gene ID | 23479 |
|---|---|
| Other Names | Iron-sulfur cluster assembly enzyme ISCU, mitochondrial, NifU-like N-terminal domain-containing protein, NifU-like protein, ISCU, NIFUN |
| Target/Specificity | This ISCU antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 138-167 amino acids from the C-terminal region of human ISCU. |
| Dilution | WB~~1:1000 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | ISCU Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | ISCU (HGNC:29882) |
|---|---|
| Synonyms | NIFUN |
| Function | [Isoform 1]: Mitochondrial scaffold protein, of the core iron-sulfur cluster (ISC) assembly complex, that provides the structural architecture on which the [2Fe-2S] clusters are assembled (PubMed:34824239). The core iron-sulfur cluster (ISC) assembly complex is involved in the de novo synthesis of a [2Fe-2S] cluster, the first step of the mitochondrial iron-sulfur protein biogenesis. This process is initiated by the cysteine desulfurase complex (NFS1:LYRM4:NDUFAB1) that produces persulfide which is delivered on the scaffold protein ISCU in a FXN-dependent manner. Then this complex is stabilized by FDX2 which provides reducing equivalents to accomplish the [2Fe-2S] cluster assembly. Finally, the [2Fe-2S] cluster is transferred from ISCU to chaperone proteins, including HSCB, HSPA9 and GLRX5 (Probable) (PubMed:24971490, PubMed:29576242, PubMed:30031876, PubMed:34824239). Exists as two slow interchanging conformational states, a structured (S) and disordered (D) form (PubMed:23940031). May modulate NFS1 desulfurase activity in a zinc-dependent manner (PubMed:30031876). Modulates the interaction between FXN and the cysteine desulfurase complex (PubMed:29576242). |
| Cellular Location | [Isoform 1]: Mitochondrion |
| Tissue Location | Detected in heart, liver, skeletal muscle, brain, pancreas, kidney, lung and placenta. |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Iron-sulfur (Fe-S) clusters are necessary for several mitochondrial enzymes and other subcellular compartment proteins. They contain sulfur and iron, and are created via several steps that include cysteine desulfurases, iron donors, chaperones, and scaffold proteins. This gene encodes the two isomeric forms, ISCU1 and ISCU2, of the Fe-S cluster scaffold protein. Mutations in this gene have been found in patients with myopathy with severe exercise intolerance and myoglobinuria.
REFERENCES
Chen, Z., et al. Oncogene 29(30):4362-4368(2010)
Chan, S.Y., et al. Cell Metab. 10(4):273-284(2009)
Kollberg, G., et al. Brain 132 (PT 8), 2170-2179 (2009) :
Huang, J., et al. J. Biol. Inorg. Chem. 13(5):825-836(2008)
Mochel, F., et al. Am. J. Hum. Genet. 82(3):652-660(2008)
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