M CCNB1 Antibody (Center S123/S125)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

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Immunohistochemical analysis of paraffin-embedded M. testis section using M CCNB1 Antibody (Center S123/S125)(Cat#AP20408c). AP20408c was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Immunohistochemical analysis of paraffin-embedded M. spleen section using M CCNB1 Antibody (Center S123/S125)(Cat#AP20408c). AP20408c was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Mouse CCNB1 Antibody (Center S123/S125)(Cat. #AP20408c) western blot analysis in mouse NIH/3T3 cell line and mouse spleen tissue lysates (35ug/lane).This demonstrates the mouse CCNB1 antibody detected the mouse CCNB1 protein (arrow).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, E
Primary Accession	P24860
Other Accession	Q08301
Reactivity	Mouse
Predicted	Hamster
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	48052 Da
Antigen Region	103-130 aa

Additional Information
Gene ID	268697
Other Names	G2/mitotic-specific cyclin-B1, Ccnb1, Ccn-2, Ccnb1-rs13, Cycb, Cycb1
Target/Specificity	This Mouse CCNB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 103-130 amino acids from the Central region of mouse CCNB1.
Dilution	WB~~1:1000 IHC-P~~1:100~500 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	M CCNB1 Antibody (Center S123/S125) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	Ccnb1
Synonyms	Ccn-2, Ccnb1-rs13, Cycb, Cycb1
Function	Essential for the control of the cell cycle at the G2/M (mitosis) transition.
Cellular Location	Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.