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HLA-B Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - HLA-B Antibody (N-term) AP20563a
    All lanes : Anti-HLA-B Antibody (N-term) at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: HL-60 whole cell lysate Lane 3: A549 whole cell lysate Lane 4: Jurkat whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 40 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - HLA-B Antibody (N-term) AP20563a
    All lanes : Anti-HLA-B Antibody (N-term) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: HL-60 whole cell lysate Lane 3: H. spleen whole lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 40 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, WB, E
Primary Accession P30464
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Additional Information
Other Names HLA class I histocompatibility antigen, B-15 alpha chain, MHC class I antigen B*15, HLA-B, HLAB
Target/Specificity This HLA-B antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 75-107 amino acids from the N-terminal region of human HLA-B.
Dilution IHC-P~~N/A
WB~~1:2000
E~~Use at an assay dependent concentration.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHLA-B Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

Involved in the presentation of foreign antigens to the immune system.

REFERENCES

Little A.-M.,et al.Tissue Antigens 38:186-190(1991).
Hildebrand W.H.,et al.Tissue Antigens 43:209-218(1994).
Lin L.,et al.Tissue Antigens 47:265-274(1996).
Madrigal J.A.,et al.J. Immunol. 149:3411-3415(1992).
Watkins D.I.,et al.Nature 357:329-333(1992).

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