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FXYD6 Antibody (C-term) 精选

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - FXYD6 Antibody (C-term) AP20610c
    All lanes : Anti-FXYD6 Antibody (C-term) at 1:1000 dilution Lane 1: Human cerebellum tissue lysate Lane 2: Human brain tissue lysate Lane 3: SH-SY5Y whole cell lysate Lane 4: Mouse brain tissue lysate Lane 5: Mouse cerebellum tissue lysate Lane 6: Rat brain tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 11 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 4 - FXYD6 Antibody (C-term) AP20610c
    Flow cytometric analysis of SH-SY5Y cells using FXYD6 Antibody (C-term)(green, Cat#AP20610c) compared to an isotype control of rabbit IgG(blue). AP20610c was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
  • 14 - FXYD6 Antibody (C-term) AP20610c
    Immunohistochemical analysis of paraffin-embedded H. liver section using FXYD6 Antibody (C-term)(Cat#AP20610c). AP20610c was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
FC, IHC-P, WB, E
Primary Accession Q9H0Q3
Reactivity Human, Rat, Mouse
Predicted Bovine, Canine, Rabbit, Chicken
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 10542 Da
Additional Information
Gene ID 53826
Other Names FXYD domain-containing ion transport regulator 6, Phosphohippolin, FXYD6
Target/Specificity This FXYD6 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 81-115amino acids from the C-terminal region of human FXYD6.
Dilution FC~~1:25
IHC-P~~1:100~500
WB~~1:1000
E~~Use at an assay dependent concentration.
Format Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsFXYD6 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name FXYD6 (HGNC:4030)
Function Associates with and regulates the activity of the sodium/potassium-transporting ATPase (NKA) which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. Reduces the apparent affinity for intracellular Na(+) with no change in the apparent affinity for extracellular K(+) (PubMed:33231612). In addition to modulating NKA kinetics, may also function as a regulator of NKA localization to the plasma membrane (By similarity).
Cellular Location Cell membrane {ECO:0000250|UniProtKB:Q91XV6}; Single-pass type I membrane protein
Research Areas

REFERENCES

Wiemann S.,et al.Genome Res. 11:422-435(2001).
Clark H.F.,et al.Genome Res. 13:2265-2270(2003).
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Brandenberger R.,et al.Nat. Biotechnol. 22:707-716(2004).
Ebert L.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.

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