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>   首页   >   产品   >   一抗   >   代谢   >   CYB5R2 Antibody (N-term)   

CYB5R2 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - CYB5R2 Antibody (N-term) AP20834a
    Western blot analysis of lysates from K562 cell line, mouse liver tissue lysate (from left to right), using CYB5R2 Antibody (N-term)(Cat. #AP20834a). AP20834a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession Q6BCY4
Reactivity Human, Rat, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 31458 Da
Additional Information
Gene ID 51700
Other Names NADH-cytochrome b5 reductase 2, b5R2, CYB5R2
Target/Specificity This CYB5R2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 33-67 amino acids from the N-terminal region of human CYB5R2.
Dilution WB~~1:1000
E~~Use at an assay dependent concentration.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCYB5R2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CYB5R2 (HGNC:24376)
Function NADH-cytochrome b5 reductases are involved in desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction (By similarity). Responsible for NADH-dependent lucigenin chemiluminescence in spermatozoa by reducing both lucigenin and 2-[4-iodophenyl]-3-[4- nitrophenyl]-5-[2,4-disulfophenyl]-2H tetrazolium monosodium salt (WST- 1).
Tissue Location Restricted expression.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

NADH-cytochrome b5 reductases are involved in desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction (By similarity). Responsible for NADH-dependent lucigenin chemiluminescence in spermatozoa by reducing both lucigenin and 2-[4-iodophenyl]-3-[4-nitrophenyl]-5-[2,4- disulfophenyl]-2H tetrazolium monosodium salt (WST-1).

REFERENCES

Zhu H.,et al.Proc. Natl. Acad. Sci. U.S.A. 96:14742-14747(1999).
Baker M.A.,et al.Biol. Reprod. 73:334-342(2005).
Bechtel S.,et al.BMC Genomics 8:399-399(2007).

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