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SNCA Antibody (C-term) 精选

Purified Rabbit Polyclonal Antibody (Pab)

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AP21113a staining SNCA in Monkey brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
AP21113a staining SNCA in Human skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Flow cytometric analysis of HeLa cells using SNCA Antibody (C-term)(green, Cat#AP21113a) compared to an isotype control of rabbit IgG(blue). AP21113a was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
Western blot analysis of lysates from Hela cell line, human brain, human cerebellum, mouse brain, rat brain tissue lysate(from left to right), using SNCA Antibody (C-term)(Cat. #AP21113a). AP21113a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, FC, E
Primary Accession	P37840
Reactivity	Human, Mouse, Rat, Cynomolgus
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	14460 Da

Additional Information
Gene ID	6622
Other Names	Alpha-synuclein, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, NACP, SNCA, NACP, PARK1
Target/Specificity	This SNCA antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 92-125 amino acids from the C-terminal region of human SNCA.
Dilution	WB~~1:1000 IHC-P~~1:100~500 FC~~1:25 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	SNCA Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	SNCA
Synonyms	NACP, PARK1
Function	Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release (PubMed:20798282, PubMed:26442590, PubMed:28288128, PubMed:30404828). Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores (PubMed:28288128, PubMed:30404828). Mechanistically, acts by increasing local Ca(2+) release from microdomains which is essential for the enhancement of ATP-induced exocytosis (PubMed:30404828). Also acts as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5 (PubMed:20798282). This chaperone activity is important to sustain normal SNARE-complex assembly during aging (PubMed:20798282). Also plays a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity (PubMed:26442590).
Cellular Location	Cytoplasm. Membrane Nucleus Synapse. Secreted. Cell projection, axon {ECO:0000250\|UniProtKB:O55042}. Note=Membrane-bound in dopaminergic neurons (PubMed:15282274). Expressed and colocalized with SEPTIN4 in dopaminergic axon terminals, especially at the varicosities (By similarity). {ECO:0000250\|UniProtKB:O55042, ECO:0000269\|PubMed:15282274}
Tissue Location	Highly expressed in presynaptic terminals in the central nervous system. Expressed principally in brain

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

May be involved in the regulation of dopamine release and transport. Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation.

REFERENCES

Ueda K.,et al.Proc. Natl. Acad. Sci. U.S.A. 90:11282-11286(1993).
Campion D.,et al.Genomics 26:254-257(1995).
Ueda K.,et al.Biochem. Biophys. Res. Commun. 205:1366-1372(1994).
Xia Y.,et al.Submitted (JAN-1996) to the EMBL/GenBank/DDBJ databases.
Touchman J.W.,et al.Genome Res. 11:78-86(2001).

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¥ 1,250.00

Cat# AP21113a

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¥ 1,250.00

¥ 2,050.00

¥ 2,960.00

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SNCA Antibody (C-term) 精选

Purified Rabbit Polyclonal Antibody (Pab)

BACKGROUND

REFERENCES

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