RHOXF1 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

All lanes : Anti-RHOXF1 Antibody (Center) at 1:2000 dilution Lane 1: A2058 whole cell lysates Lane 2: human ovary lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 21 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes : Anti-RHOXF1 Antibody (Center) at 1:1000 dilution Lane 1: HL-60 whole cell lysates Lane 2: human testis lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 21 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, E
Primary Accession	Q8NHV9
Reactivity	Human
Host	Rabbit
Clonality	polyclonal
Isotype	Rabbit IgG
Calculated MW	20542 Da

Additional Information
Gene ID	158800
Other Names	Rhox homeobox family member 1, Ovary-, testis- and epididymis-expressed gene protein, Paired-like homeobox protein PEPP-1, RHOXF1, OTEX, PEPP1
Target/Specificity	This RHOXF1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 86-120 amino acids from the Central region of human RHOXF1.
Dilution	WB~~1:1000 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	RHOXF1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	RHOXF1 (HGNC:29993)
Synonyms	OTEX, PEPP1
Function	Transcription factor maybe involved in reproductive processes. Modulates expression of target genes encoding proteins involved in processes relevant to spermatogenesis.
Cellular Location	Nucleus {ECO:0000255\|PROSITE-ProRule:PRU00108, ECO:0000269\|PubMed:11980563}
Tissue Location	Ovary, testis and epididymis. Also detected in the prostate and the mammary gland. Expressed in many tumor cell lines derived from acute lymphocytic leukemia, prostate, endometrial adenocarcinoma, melanoma, bladder carcinoma, colon carcinoma, erythroleukemia and breast carcinoma. Not expressed in placenta. In testis, mainly expressed in germ cells, but also detected in somatic cells such as Sertoli cells, Leydig cells and peritubular cells (PubMed:28171660).

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.