癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
(Mouse) Pou5f1 Antibody (N-term)
Purified Rabbit Polyclonal Antibody (Pab)
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- 实验流程
- 背景知识
Application 
| WB, IHC-P, E |
|---|---|
| Primary Accession | P20263 |
| Reactivity | Mouse |
| Host | Rabbit |
| Clonality | polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 38216 Da |
| Gene ID | 18999 |
|---|---|
| Other Names | POU domain, class 5, transcription factor 1, NF-A3, Octamer-binding protein 3, Oct-3, Octamer-binding protein 4, Oct-4, Octamer-binding transcription factor 3, OTF-3, Pou5f1, Oct-3, Oct-4, Otf-3, Otf3 |
| Target/Specificity | This Mouse Pou5f1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 86-119 amino acids from the N-terminal region of Mouse Pou5f1. |
| Dilution | WB~~1:2000 IHC-P~~1:100~500 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | (Mouse) Pou5f1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | Pou5f1 |
|---|---|
| Synonyms | Oct-3, Oct-4, Otf-3, Otf3 |
| Function | Transcription factor that binds to the octamer motif (5'- ATTTGCAT-3') (PubMed:1690859, PubMed:17525163, PubMed:1967980, PubMed:1972777, PubMed:23376973). Forms a trimeric complex with SOX2 or SOX15 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (PubMed:15863505, PubMed:17097055, PubMed:17496161, PubMed:19740739). Critical for early embryogenesis and for embryonic stem cell pluripotency (PubMed:1690859, PubMed:17496161, PubMed:18662995, PubMed:1972777, PubMed:19740739, PubMed:23376973, PubMed:29153991, PubMed:32703285). |
| Cellular Location | Cytoplasm {ECO:0000250|UniProtKB:Q01860}. Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108, ECO:0000255|PROSITE- ProRule:PRU00530, ECO:0000269|PubMed:17496161, ECO:0000269|PubMed:17525163, ECO:0000269|PubMed:29153991} Note=Expressed in a diffuse and slightly punctuate pattern (By similarity). Colocalizes with MAPK8 and MAPK9 in the nucleus (PubMed:29153991). {ECO:0000250|UniProtKB:Q01860, ECO:0000269|PubMed:29153991} |
| Tissue Location | Expressed the totipotent and pluripotent stem cells of the pregastrulation embryo. Also expressed in primordial germ cells and in the female germ line. Absent from adult tissues |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.
REFERENCES
Rosner M.H.,et al.Nature 345:686-692(1990).
Schoeler H.R.,et al.Nature 344:435-439(1990).
Okamoto K.,et al.Cell 60:461-472(1990).
Okazawa H.,et al.EMBO J. 10:2997-3005(1991).
Tsuruzoe S.,et al.Biochem. Biophys. Res. Commun. 351:920-926(2006).
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