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DYNC1H1 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

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All lanes : Anti-DYNC1H1 Antibody (C-term) at 1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: PC-3 whole cell lysate Lane 3: U-87 MG whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 532 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes : Anti-DYNC1H1 Antibody (C-term) at 1:2000 dilution Lane 1: mouse brain lysate Lane 2: PC-3 whole cell lysate Lane 3: U-87 MG whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 532 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes : Anti-DYNC1H1 Antibody (C-term) at 1:1000-1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: PC-3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 532 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes : Anti-DYNC1H1 Antibody (C-term) at 1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: PC-3 whole cell lysate Lane 3: mouse brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 532 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
AP21385b staining DYNC1H1 in human testis sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
All lanes : Anti-DYNC1H1 Antibody (C-term) at 1:2000 dilution Lane 1: U-87 MG whole cell lysates Lane 2: PC-3 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 532 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, E
Primary Accession	Q14204
Reactivity	Human, Rat, Mouse
Host	Rabbit
Clonality	polyclonal
Isotype	Rabbit IgG
Calculated MW	532408 Da

Additional Information
Gene ID	1778
Other Names	Cytoplasmic dynein 1 heavy chain 1, Cytoplasmic dynein heavy chain 1, Dynein heavy chain, cytosolic, DYNC1H1, DHC1, DNCH1, DNCL, DNECL, DYHC, KIAA0325
Target/Specificity	This DYNC1H1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 4202-4236 amino acids from the C-terminal region of human DYNC1H1.
Dilution	WB~~1:2000 IHC-P~~1:100~500 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	DYNC1H1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	DYNC1H1 (HGNC:2961)
Function	Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP. Plays a role in mitotic spindle assembly and metaphase plate congression (PubMed:27462074).
Cellular Location	Cytoplasm, cytoskeleton

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP.

REFERENCES

Nagase T.,et al.DNA Res. 4:141-150(1997).
Ohara O.,et al.Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
Yamakawa H.,et al.Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases.
Vaisberg E.A.,et al.J. Cell Biol. 133:831-842(1996).
Vaisberg E.A.,et al.J. Cell Biol. 123:849-858(1993).

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¥ 1,250.00

Cat# AP21385b

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50 µl

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¥ 1,250.00

¥ 2,050.00

¥ 2,960.00

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DYNC1H1 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

BACKGROUND

REFERENCES

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