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HNRNPUL2 Antibody (Center) 精选

Purified Rabbit Polyclonal Antibody (Pab)

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AP21566c staining HNRNPUL2 in human stomach tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
All lanes : Anti-HNRNPUL2 Antibody (Center) at 1:2000 dilution Lane 1: MCF-7 whole cell lysates Lane 2: Jurkat whole cell lysates Lane 3: 293T/17 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 85 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-HNRNPUL2 Antibody (Center)at 1:8000 dilution + Hela whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 85 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, E
Primary Accession	Q1KMD3
Reactivity	Human, Mouse
Predicted	Rat, Bovine, Canine, Rabbit
Host	Rabbit
Clonality	polyclonal
Isotype	Rabbit IgG
Calculated MW	85105 Da

Additional Information
Gene ID	221092
Other Names	Heterogeneous nuclear ribonucleoprotein U-like protein 2, Scaffold-attachment factor A2, SAF-A2, HNRNPUL2, HNRPUL2
Target/Specificity	This HNRNPUL2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 337-350 amino acids from the Central region of human HNRNPUL2.
Dilution	WB~~1:8000 IHC-P~~1:100~500 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	HNRNPUL2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	HNRNPUL2
Synonyms	HNRPUL2
Cellular Location	Nucleus {ECO:0000250\|UniProtKB:Q00PI9}.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Herrmann F.,et al.Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases.
Bechtel S.,et al.BMC Genomics 8:399-399(2007).
Bienvenut W.V.,et al.Submitted (DEC-2008) to UniProtKB.
Burkard T.R.,et al.BMC Syst. Biol. 5:17-17(2011).

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¥ 1,250.00

Cat# AP21566c

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¥ 1,250.00

¥ 2,050.00

¥ 2,960.00

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HNRNPUL2 Antibody (Center) 精选

Purified Rabbit Polyclonal Antibody (Pab)

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