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TMEM97 Antibody (N-term) 精选
Purified Rabbit Polyclonal Antibody (Pab)
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, E |
|---|---|
| Primary Accession | Q5BJF2 |
| Reactivity | Human, Rat, Mouse |
| Predicted | Canine, Rabbit |
| Host | Rabbit |
| Clonality | polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 20848 Da |
| Gene ID | 27346 |
|---|---|
| Other Names | Transmembrane protein 97, Protein MAC30, TMEM97, MAC30 |
| Target/Specificity | This TMEM97 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 22-56 amino acids from the N-terminal region of human TMEM97. |
| Dilution | WB~~1：1000 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | TMEM97 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | TMEM97 (HGNC:28106) |
|---|---|
| Function | Sigma-2 receptor implicated in the regulation of multiple cellular processes, including cholesterol biosynthesis and trafficking, membrane trafficking, autophagy, lipid membrane-associated protein trafficking, and stabilization of receptors at the cell surface, thereby contributing to the maintenance of cellular homeostasis(PubMed:19583955, PubMed:23922215, PubMed:25620095, PubMed:27378690, PubMed:28559337, PubMed:30443021, PubMed:34233061, PubMed:34799735, PubMed:35970844, PubMed:25566323). Forms a ternary complex with PGRMC1 and LDLR at the plasma membrane, promoting LDLR- mediated internalization of LDL cholesterol (PubMed:30443021). Reduces the availability of the lysosomal sterol transporter NPC1, likely through direct interaction, thereby regulating lipid transport, including cholesterol and LBPA, outside of late endosome/lysosome (PubMed:19583955, PubMed:27378690). Binds the regio- and stereoselective oxysterol 20(S)-hydroxycholesterol (20(S)-OHC). Ligand binding enhances association with NPC1 and modulates TMEM97-containing protein complexes involving PGRMC1 and TSPO, consistent with a role in cholesterol homeostasis (PubMed:34799735, PubMed:37047353). Also able to bind cholesterol (By similarity). Interacts with the salivary peptide histatin 1 (Hst 1/HTN1) at the ER membrane, which is critical for increasing mitochondria-ER contacts and stimulating Hst 1 wound healing properties (PubMed:34233061, PubMed:35970844). May alter the activity of some cytochrome P450 proteins (PubMed:22292588). Although sharing homology with sterol isomerases through the EXPERA domain, lacks sterol isomerase activity; instead, may function as a sterol or oxysterol sensor (PubMed:34880501, PubMed:25566323). Acts as an ER quality control factor by promoting proteolytic degradation of nonproductive and extramitochondrial precursor proteins in the ER membrane, thereby removing them from the ER surface (By similarity). |
| Cellular Location | Rough endoplasmic reticulum membrane; Multi-pass membrane protein. Nucleus membrane; Multi- pass membrane protein. Note=Localized at cell membrane and in lysosomes in sterol-depleted cells when expression of endogenous TMEM97 is stimulated (PubMed:19583955). Localized at cell membrane, probably in lipid rafts, in serum-starved conditions (PubMed:30443021) |
| Tissue Location | Widely expressed in normal tissues. Expressed in pancreatic, renal, breast, colon, ovarian surface epithelial (OSE) cells. Highly expressed in various proliferating cancer cells (PubMed:23922215). |
Research Areas
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Plays a role as a regulator of cellular cholesterol homeostasis.
REFERENCES
Murphy M.,et al.Cell Growth Differ. 4:715-722(1993).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Kayed H.,et al.Histol. Histopathol. 19:1021-1031(2004).
Wilcox C.B.,et al.BMC Cancer 7:223-223(2007).
Bartz F.,et al.Cell Metab. 10:63-75(2009).
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¥ 1,250.00
Cat# AP21745a
