beta Tubulin

Purified Rabbit Polyclonal Antibody (Pab)

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AP22105a staining beta Tubulin in mouse thymus tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Overlay histogram showing C2C12 cells stained with AP22105a(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22105a, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
All lanes : Anti-beta Tubulin at 1:2000 dilution Lane 1: C2C12 whole cell lysate Lane 2: L6 whole cell lysate Lane 3: mouse brain lysate Lane 4: mouse spleen whole cell lysate Lane 5: mouse thymus lysate Lane 6: NIH/3T3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 50 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

产品详情
文献引用 : 2
实验流程
背景知识

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, FC, IHC-P, E
Primary Accession	P99024
Other Accession	Q17299, P12456, P09203, Q24560, Q9YHC3, Q27U48, O17449, P36221, Q6EVK8, Q13885, Q4R5B3, Q7TMM9, P85108, Q6B856, Q9BVA1, Q9CWF2, Q3KRE8, P52275, P32882, P83130, P61858, P61857, P13602, Q2T9S0, P09206, Q13509, Q60HC2, Q9ERD7, Q4QRB4, Q3ZBU7, P04350, Q4R4X8, Q9D6F9, Q3MHM5, P68371, P86221, P68
Reactivity	Rat, Mouse
Predicted	Bovine, Chicken, Human, Mouse, Pig, Rat
Host	Rabbit
Clonality	polyclonal
Isotype	Rabbit IgG

Additional Information
Other Names	Tubulin beta-5 chain, Tubb5
Target/Specificity	This antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 46-78 amino acids from human.
Dilution	WB~~1:2000 FC~~1:25 IHC-P~~1:100~500 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	beta Tubulin is for research use only and not for use in diagnostic or therapeutic procedures.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

REFERENCES

Wang D.,et al.J. Cell Biol. 103:1903-1910(1986).
Carninci P.,et al.Science 309:1559-1563(2005).
Church D.M.,et al.PLoS Biol. 7:E1000112-E1000112(2009).
Lubec G.,et al.Submitted (JUL-2007) to UniProtKB.
Lewis S.A.,et al.J. Cell Biol. 101:852-861(1985).

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¥ 1,250.00

Cat# AP22105a

Size:

50 µl

100 µl

200 µl

400 µl

Price:

¥ 1,250.00

¥ 2,050.00

¥ 2,960.00

¥ 4,550.00

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Availability: In Stock

询价

beta Tubulin

Purified Rabbit Polyclonal Antibody (Pab)

BACKGROUND

REFERENCES

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