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EEF1A1P5 Antibody (C-Term)

Purified Rabbit Polyclonal Antibody (Pab)

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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-2 OS (human osteosarcoma cell line) cells labeling EEF1A1P5 with AP22206b at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG (NK179883) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on U-2 OS cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (PD18466410) at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).
Overlay histogram showing HepG2 cells stained with AP22206b(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22206b, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. .
All lanes : Anti-EEF1A1P5 Antibody (C-Term) at 1:1000-1:2000 dilution Lane 1: U-2OS whole cell lysate Lane 2: 293 whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: MCF-7 whole cell lysate Lane 5: PC-3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 50 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, FC, IF, E
Primary Accession	Q5VTE0
Other Accession	P68103, P62629, Q66RN5, A2Q0Z0, P68104, P10126, Q5R1X2, Q5R4R8, P68105, P62630, Q90835
Reactivity	Human
Predicted	Bovine, Chicken, Human, Mouse, Rat, Horse
Host	Rabbit
Clonality	polyclonal
Isotype	Rabbit IgG
Calculated MW	50185 Da

Additional Information
Other Names	Putative elongation factor 1-alpha-like 3, EF-1-alpha-like 3, Eukaryotic elongation factor 1 A-like 3, eEF1A-like 3, Eukaryotic translation elongation factor 1 alpha-1 pseudogene 5, EEF1A1P5, EEF1AL3
Target/Specificity	This EEF1A1P5 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 430-462 amino acids from human EEF1A1P5.
Dilution	WB~~1:1000-1:2000 FC~~1:25 IF~~1:25 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	EEF1A1P5 Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	EEF1A1P5
Synonyms	EEF1AL3
Function	This protein promotes the GTP-dependent binding of aminoacyl- tRNA to the A-site of ribosomes during protein biosynthesis.
Cellular Location	Cytoplasm.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.

REFERENCES

Humphray S.J.,et al.Nature 429:369-374(2004).
Bienvenut W.V.,et al.Submitted (DEC-2008) to UniProtKB.
Bienvenut W.V.,et al.Submitted (MAR-2009) to UniProtKB.
Li W.B.,et al.Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases.
Lund A.,et al.Genomics 36:359-361(1996).

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¥ 1,250.00

Cat# AP22206b

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EEF1A1P5 Antibody (C-Term)

Purified Rabbit Polyclonal Antibody (Pab)

BACKGROUND

REFERENCES

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