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>   首页   >   产品   >   一抗   >   癌症   >   AGL Antibody (Center)   

AGL Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - AGL Antibody (Center) AP2402a
    Western blot analysis of hAGL-C371 (Cat. #AP2402a) in K562 cell line lysates (35ug/lane). AGL (arrow) was detected using the purified Pab.
  • 3 - AGL Antibody (Center) AP2402a
    Confocal immunofluorescent analysis of AGL Antibody (Center)(Cat#AP2402a) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IF, E
Primary Accession P35573
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 174764 Da
Antigen Region 357-387 aa
Additional Information
Gene ID 178
Other Names Glycogen debranching enzyme, Glycogen debrancher, 4-alpha-glucanotransferase, Oligo-1, 4-1, 4-glucantransferase, Amylo-alpha-1, 6-glucosidase, Amylo-1, 6-glucosidase, Dextrin 6-alpha-D-glucosidase, AGL, GDE
Target/Specificity This AGL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 357-387 amino acids from the Central region of human AGL.
Dilution WB~~1:1000
IF~~1:10~50
E~~Use at an assay dependent concentration.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAGL Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name AGL
Synonyms GDE
Function Multifunctional enzyme acting as 1,4-alpha-D-glucan:1,4- alpha-D-glucan 4-alpha-D-glycosyltransferase and amylo-1,6-glucosidase in glycogen degradation.
Cellular Location Cytoplasm. Note=Under glycogenolytic conditions localizes to the nucleus
Tissue Location Liver, kidney and lymphoblastoid cells express predominantly isoform 1; whereas muscle and heart express not only isoform 1, but also muscle-specific isoform mRNAs (isoforms 2, 3 and 4). Isoforms 5 and 6 are present in both liver and muscle
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

AGL is a glycogen debrancher enzyme which is involved in glycogen degradation. This enzyme has two independent catalytic activities which occur at different sites on the protein: a 4-alpha-glucotransferase activity and a amylo-1,6-glucosidase activity. Mutations in the AGL gene are associated with glycogen storage disease although a wide range of enzymatic and clinical variability occurs which may be due to tissue-specific alternative splicing.

REFERENCES

Horinishi, A., et al., J. Hum. Genet. 47(2):55-59 (2002).
Bao, Y., et al., Genomics 38(2):155-165 (1996).
Yang, B.Z., et al., J. Biol. Chem. 267(13):9294-9299 (1992).
Yang-Feng, T.L., et al., Genomics 13(4):931-934 (1992).
Bao, Y., et al., Gene 197 (1-2), 389-398 (1997).

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