癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
EZH1 Antibody (Center)
Purified Rabbit Polyclonal Antibody (Pab)
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, IHC-P, E |
|---|---|
| Primary Accession | Q92800 |
| Other Accession | A7E2Z2 |
| Reactivity | Human |
| Predicted | Bovine |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 85271 Da |
| Antigen Region | 393-422 aa |
| Gene ID | 2145 |
|---|---|
| Other Names | Histone-lysine N-methyltransferase EZH1, ENX-2, Enhancer of zeste homolog 1, EZH1, KIAA0388 |
| Target/Specificity | This EZH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 393-422 amino acids from the Central region of human EZH1. |
| Dilution | WB~~1:1000 IHC-P~~1:100~500 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | EZH1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | EZH1 |
|---|---|
| Synonyms | KIAA0388 |
| Function | Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH1 complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Required for embryonic stem cell derivation and self-renewal, suggesting that it is involved in safeguarding embryonic stem cell identity. Compared to EZH2-containing complexes, it is less abundant in embryonic stem cells, has weak methyltransferase activity and plays a less critical role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. |
| Cellular Location | Nucleus. Note=Colocalizes with trimethylated 'Lys-27' of histone H3 |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
EZH1 encodes a protein of 747 amino acids that displays 55% amino acid identity overall with the Drosophila homolog.1 The strong sequence conservation suggested potential roles for EZH1 in human development as a transcriptional regulator and as a component of protein complexes that preserve heterochromatin stability. EZH1 is expressed as 2 major transcripts in all adult and fetal human tissues evaluated.. Analysis of an EZH1 cDNA revealed an unusual splicing event involving EZH1 and a tandemly linked gene GPR2 and suggested a potential mechanism for modifying the EZH1 protein in the conserved C-terminal domain. The GPR2 gene maps to 17q21.1-q21.3 in the vicinity of the BRCA1 gene.
REFERENCES
Ogawa, M., et al., Biochim. Biophys. Acta 1395(2):151-158 (1998).
Abel, K.J., et al., Genomics 37(2):161-171 (1996).
Friedman, L.S., et al., Genomics 25(1):256-263 (1995).
Osborne-Lawrence, S., et al., Genomics 25(1):248-255 (1995).
Brody, L.C., et al., Genomics 25(1):238-247 (1995).
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