ANGEL2 Antibody (Center) 精选

Purified Rabbit Polyclonal Antibody (Pab)

Western blot analysis of anti-ANGEL2 Pab (Cat.#AP2749c) in CEM cell line lysates (35ug/lane). ANGEL2 (arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with ANGEL2 antibody (Center) (Cat.#AP2749c), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, E
Primary Accession	Q5VTE6
Reactivity	Human
Predicted	Mouse, Rat, Bovine, Canine
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	62339 Da
Antigen Region	108-138 aa

Additional Information
Gene ID	90806
Other Names	Protein angel homolog 2, ANGEL2, KIAA0759L
Target/Specificity	This ANGEL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 108-138 amino acids from the Central region of human ANGEL2.
Dilution	WB~~1:1000 IHC-P~~1:100~500 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	ANGEL2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	ANGEL2
Synonyms	KIAA0759L
Function	RNA 2',3'-cyclic phosphatase that catalyzes the hydrolysis of RNA molecules with a 2',3'-cyclic phosphate at the 3' end to produce RNA with a linear 3'-phosphate group (PubMed:32732418, PubMed:36180430). Essential for the maturation and processing of mitochondrial RNAs, particularly those generated via non-canonical pathways (By similarity). Antagonizes pre-tRNA processing and XBP1 mRNA splicing during the unfolded protein response by limiting the availability of 2',3'-cyclic phosphate substrates for the tRNA ligase complex (PubMed:32732418). Promotes tRNA recycling by eliminating 2',3'-cyclic phosphate groups from cleaved tRNAs, thereby enabling the tRNA nucleotidyltransferase TRNT1 to reattach the CCA tail essential for aminoacylation and subsequent translation (PubMed:32732418).
Cellular Location	Mitochondrion matrix