STIP1 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

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STIP1 Antibody (Center) (Cat. #AP2817c) western blot analysis in A431,A549 cell line lysates (35ug/lane).This demonstrates the STIP1 antibody detected the STIP1 protein (arrow).
Formalin-fixed and paraffin-embedded human testis tissue reacted with CNOT4 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
STIP1 Antibody (Center) (Cat. #AP2817c) flow cytometry analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IHC-P, FC, WB, E
Primary Accession	P31948
Other Accession	O35814, Q60864, O54981, Q3ZBZ8
Reactivity	Human, Rat, Mouse
Predicted	Bovine, Hamster, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	62639 Da
Antigen Region	269-297 aa

Additional Information
Gene ID	10963
Other Names	Stress-induced-phosphoprotein 1, STI1, Hsc70/Hsp90-organizing protein, Hop, Renal carcinoma antigen NY-REN-11, Transformation-sensitive protein IEF SSP 3521, STIP1
Target/Specificity	This STIP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 269-297 amino acids from the Central region of human STIP1.
Dilution	IHC-P~~1:100~500 FC~~1:10~50 WB~~1:1000 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	STIP1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	STIP1 (HGNC:11387)
Function	Acts as a co-chaperone for HSP90AA1 (PubMed:27353360). Mediates the association of the molecular chaperones HSPA8/HSC70 and HSP90 (By similarity).
Cellular Location	Cytoplasm {ECO:0000250\|UniProtKB:Q60864}. Nucleus {ECO:0000250\|UniProtKB:Q60864}. Dynein axonemal particle {ECO:0000250\|UniProtKB:Q7ZWU1}

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.