STX3 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

Formalin-fixed and paraffin-embedded human brain tissue reacted with STX3 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of anti-STX3 Antibody (Center) (Cat.#AP2818c) in A375 cell line lysates (35ug/lane). STX3(arrow) was detected using the purified Pab.
Western blot analysis of STX3 (arrow) using rabbit polyclonal STX3 Antibody (Center) (Cat.#AP2818c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the STX3 gene.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IHC-P, WB, E
Primary Accession	Q13277
Other Accession	Q08849, Q64704
Reactivity	Human
Predicted	Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	33155 Da
Antigen Region	178-205 aa

Additional Information
Gene ID	6809
Other Names	Syntaxin-3, STX3, STX3A
Target/Specificity	This STX3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 178-205 amino acids from the Central region of human STX3.
Dilution	IHC-P~~1:100~500 WB~~1:1000 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	STX3 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	STX3
Synonyms	STX3A
Function	Potentially involved in docking of synaptic vesicles at presynaptic active zones. Apical receptor involved in membrane fusion of apical vesicles.
Cellular Location	[Isoform A]: Apical cell membrane; Single-pass type IV membrane protein. Note=Localized to the inner and outer plexiform layers, the cell body and the inner segments of photoreceptors {ECO:0000250\|UniProtKB:Q64704}
Tissue Location	[Isoform A]: Expressed in small intestine, kidney, pancreas, placenta as well as in retina. Weaker expression in lung, liver and heart. Not expressed in brain and skeletal muscle [Isoform 3]: Ubiquitously expressed.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.