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DDAH1 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

Formalin-fixed and paraffin-embedded mouse kidney reacted with DDAH1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of DDAH1 Antibody (C-term) (Cat. #AP2898b) in mouse kidney tissue lysates (35ug/lane). DDAH1 (arrow) was detected using the purified Pab.
DDAH1 Antibody (C-term) (Cat. #AP2898b) flow cytometric analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Confocal immunofluorescent analysis of DDAH1 Antibody (C-term)(Cat#AP2898b) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

产品详情
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实验流程
背景知识

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IHC-P, WB, FC, IF, E
Primary Accession	O94760
Other Accession	O08557, Q9CWS0, P56965
Reactivity	Human, Mouse
Predicted	Bovine, Rat
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	31122 Da
Antigen Region	221-250 aa

Additional Information
Gene ID	23576
Other Names	N(G), N(G)-dimethylarginine dimethylaminohydrolase 1, DDAH-1, Dimethylarginine dimethylaminohydrolase 1, DDAHI, Dimethylargininase-1, DDAH1, DDAH
Target/Specificity	This DDAH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 221-250 amino acids from the C-terminal region of human DDAH1.
Dilution	IHC-P~~1:100~500 WB~~1:1000 FC~~1:10~50 IF~~1:10~50 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	DDAH1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	DDAH1 (HGNC:2715)
Synonyms	DDAH
Function	Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)- monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation.
Tissue Location	Detected in brain, liver, kidney and pancreas, and at low levels in skeletal muscle.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

DDAH1 plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity.

REFERENCES

Ellger,B., et.al., Endocrinology 149 (6), 3148-3157 (2008)

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Discontinued

Cat# AP2898B

Size:

50 µl

100 µl

200 µl

400 µl

Price:

¥ 1,250.00

¥ 2,050.00

¥ 2,960.00

¥ 4,550.00

替代产品:

DDAH1 Antibody, DDAH1 Rabbit mAb,

DDAH1 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

BACKGROUND

REFERENCES

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