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IMMT Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - IMMT Antibody (Center) AP2931c
    Western blot analysis of IMMT Antibody (Center) (Cat. #AP2931c) in Hela, NCI-H460, CEM cell line lysates (35ug/lane). IMMT (arrow) was detected using the purified Pab.
  • 14 - IMMT Antibody (Center) AP2931c
    Formalin-fixed and paraffin-embedded human brain tissue reacted with IMMT Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • 4 - IMMT Antibody (Center) AP2931c
    IMMT Antibody (Center) (Cat. #AP2931c) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of IMMT Antibody (Center) (Cat. #AP2931c) in Hela, NCI-H460, CEM cell line lysates (35ug/lane). IMMT (arrow) was detected using the purified Pab.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, FC, E
Primary Accession Q16891
Other Accession Q3KR86, Q8CAQ8
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 83678 Da
Antigen Region 438-467 aa
Additional Information
Gene ID 10989
Other Names MICOS complex subunit MIC60, Cell proliferation-inducing gene 4/52 protein, Mitochondrial inner membrane protein, Mitofilin, p87/89, IMMT, HMP, MIC60, MINOS2
Target/Specificity This IMMT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 438-467 amino acids from the Central region of human IMMT.
Dilution WB~~1:1000
IHC-P~~1:100~500
FC~~1:10~50
E~~Use at an assay dependent concentration.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsIMMT Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name IMMT
Synonyms HMP, MIC60, MINOS2
Function Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane (PubMed:22114354, PubMed:25781180, PubMed:32567732, PubMed:33130824). Plays an important role in the maintenance of the MICOS complex stability and the mitochondrial cristae morphology (PubMed:22114354, PubMed:25781180, PubMed:32567732, PubMed:33130824).
Cellular Location Mitochondrion inner membrane; Single-pass membrane protein. Mitochondrion
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

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