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>   首页   >   产品   >   一抗   >   精选抗体   >   磷酸化抗体   >   Phospho-BACE(S498) Antibody   

Phospho-BACE(S498) Antibody

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - Phospho-BACE(S498) Antibody AP3032a
    Dot blot analysis of Phospho-BACE(S498) Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antobodies working concentration was 0. 5ug per ml.
  • 14 - Phospho-BACE(S498) Antibody AP3032a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession P56817
Other Accession P56819, P56818, Q2HJ40
Reactivity Human
Predicted Mouse, Rat, Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 55764 Da
Additional Information
Gene ID 23621
Other Names Beta-secretase 1, Aspartyl protease 2, ASP2, Asp 2, Beta-site amyloid precursor protein cleaving enzyme 1, Beta-site APP cleaving enzyme 1, Memapsin-2, Membrane-associated aspartic protease 2, BACE1, BACE, KIAA1149
Target/Specificity This BACE Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S498 of human BACE.
Dilution WB~~1:1000
IHC-P~~1:100~500
E~~Use at an assay dependent concentration.
Format Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPhospho-BACE(S498) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name BACE1 (HGNC:933)
Synonyms BACE, KIAA1149
Function Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase (PubMed:10656250, PubMed:10677483, PubMed:20354142). Cleaves CHL1 (By similarity).
Cellular Location Cell membrane; Single-pass type I membrane protein Golgi apparatus, trans-Golgi network. Endoplasmic reticulum. Endosome. Cell surface. Cytoplasmic vesicle membrane; Single-pass type I membrane protein. Membrane raft {ECO:0000250|UniProtKB:P56818}. Lysosome. Late endosome. Early endosome. Recycling endosome. Cell projection, axon {ECO:0000250|UniProtKB:P56818}. Cell projection, dendrite {ECO:0000250|UniProtKB:P56818}. Note=Predominantly localized to the later Golgi/trans-Golgi network (TGN) and minimally detectable in the early Golgi compartments. A small portion is also found in the endoplasmic reticulum, endosomes and on the cell surface (PubMed:11466313, PubMed:17425515). Colocalization with APP in early endosomes is due to addition of bisecting N-acetylglucosamine which blocks targeting to late endosomes and lysosomes (By similarity) Retrogradly transported from endosomal compartments to the trans-Golgi network in a phosphorylation- and GGA1- dependent manner (PubMed:15886016). {ECO:0000250|UniProtKB:P56818, ECO:0000269|PubMed:11466313, ECO:0000269|PubMed:15886016, ECO:0000269|PubMed:17425515}
Tissue Location Expressed at high levels in the brain and pancreas. In the brain, expression is highest in the substantia nigra, locus coruleus and medulla oblongata.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

Cerebral deposition of amyloid beta peptide is an early and critical feature of Alzheimer's disease. Amyloid beta peptide is generated by proteolytic cleavage of amyloid precursor protein (APP) by two proteases, one of which is BACE. The encoded protein, a member of the peptidase A1 protein family, is a type I integral membrane glycoprotein and aspartic protease that is found mainly in the Golgi.

REFERENCES

Xie, J., et al., J. Biol. Chem. 280(14):13824-13832 (2005).
He, X., et al., J. Biol. Chem. 280(12):11696-11703 (2005).
Huang, X.P., et al., J. Biol. Chem. 279(36):37886-37894 (2004).
Chiocco, M.J., et al., J. Biol. Chem. 279(50):52535-52542 (2004).
Yang, H.C., et al., J. Neurochem. 91(6):1249-1259 (2004).

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