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Phospho-CDC25A(S293) Antibody

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - Phospho-CDC25A(S293) Antibody AP3048a
    All lanes: Anti-Cdc25A Antibody (S292) at 1:1000 dilution Lane 1: MCF-7 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ASP1615) at 1/15000 dilution. Observed band size: 59KDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - Phospho-CDC25A(S293) Antibody AP3048a
    The anti-Phospho-CDC25A-S293 Pab (Cat. #AP3048a) is used in Western blot to detect Phospho-CDC25A-S293 in cells transfected with wild type or mutant S293A of CDC25A. Data courtesy of Dr. Tiebang Kang of Washington University, St. Louis, MO.
  • 14 - Phospho-CDC25A(S293) Antibody AP3048a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
All lanes: Anti-Cdc25A Antibody (S292) at 1:1000 dilution Lane 1: MCF-7 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ASP1615) at 1/15000 dilution. Observed band size: 59KDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession P30304
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 59087 Da
Additional Information
Gene ID 993
Other Names M-phase inducer phosphatase 1, Dual specificity phosphatase Cdc25A, CDC25A
Target/Specificity This CDC25A Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S293 of human CDC25A.
Dilution WB~~1:1000
IHC-P~~1:100~500
E~~Use at an assay dependent concentration.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPhospho-CDC25A(S293) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CDC25A
Function Tyrosine protein phosphatase which functions as a dosage- dependent inducer of mitotic progression (PubMed:12676925, PubMed:14559997, PubMed:1836978, PubMed:20360007). Directly dephosphorylates CDK1 and stimulates its kinase activity (PubMed:20360007). Also dephosphorylates CDK2 in complex with cyclin-E, in vitro (PubMed:20360007).
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

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