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>   首页   >   产品   >   一抗   >   精选抗体   >   磷酸化抗体   >   Phospho-p27Kip1(S10) Antibody   

Phospho-p27Kip1(S10) Antibody

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - Phospho-p27Kip1(S10) Antibody AP3191a
    The anti-Phospho-p27Kip1-S10 Pab (Cat. #AP9191a) is used in Western blot to detect Phospho-p27Kip1-S10 in HL60 tissue lysate
  • 14 - Phospho-p27Kip1(S10) Antibody AP3191a
    Formalin-fixed and paraffin-embedded human skeletal muscle with Phospho-p27Kip1-S10 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession P46527
Other Accession P46414, Q60439
Reactivity Human
Predicted Mouse, Hamster
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 22073 Da
Additional Information
Gene ID 1027
Other Names Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1, CDKN1B, KIP1
Target/Specificity This p27Kip1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S10 of human p27Kip1.
Dilution WB~~1:1000
IHC-P~~1:100~500
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPhospho-p27Kip1(S10) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CDKN1B {ECO:0000303|PubMed:20824794}
Function Important regulator of cell cycle progression. Inhibits the kinase activity of CDK2 bound to cyclin A, but has little inhibitory activity on CDK2 bound to SPDYA (PubMed:28666995). Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.
Cellular Location Nucleus. Cytoplasm. Endosome. Note=Nuclear and cytoplasmic in quiescent cells. AKT- or RSK-mediated phosphorylation on Thr-198, binds 14-3-3, translocates to the cytoplasm and promotes cell cycle progression. Mitogen-activated UHMK1 phosphorylation on Ser-10 also results in translocation to the cytoplasm and cell cycle progression. Phosphorylation on Ser-10 facilitates nuclear export. Translocates to the nucleus on phosphorylation of Tyr-88 and Tyr-89. Colocalizes at the endosome with SNX6; this leads to lysosomal degradation (By similarity)
Tissue Location Expressed in kidney (at protein level) (PubMed:15509543). Expressed in all tissues tested (PubMed:8033212) Highest levels in skeletal muscle, lowest in liver and kidney (PubMed:8033212).
Research Areas

BACKGROUND

p27Kip1 is a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.

REFERENCES

Kawamata, N., et al., Eur. J. Haematol. 74(5):424-429 (2005). Andreu, E.J., et al., Cancer Res. 65(8):3264-3272 (2005). Wingate, H., et al., J. Biol. Chem. 280(15):15148-15157 (2005). Wang, C., et al., J. Biol. Chem. 280(13):12339-12343 (2005). Rassidakis, G.Z., et al., Blood 105(2):827-829 (2005).

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