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>   首页   >   产品   >   一抗   >   精选抗体   >   磷酸化抗体   >   Phospho-TNIK(S764) Antibody   

Phospho-TNIK(S764) Antibody

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - Phospho-TNIK(S764) Antibody AP3276A
    Dot blot analysis of anti-TNIK(S764) Antibody Phospho-specific Pab (Cat. #AP3276a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0. 5ug per ml.
  • 6 - Phospho-TNIK(S764) Antibody AP3276A
    Dot blot analysis of Phospho-TNIK-pS764 Pab (Cat. #AP3276a) and TNIK-pS764 Pab (AP7970d)on nitrocellulose membrane. 50ng of Phospho-peptide (BP3276a) or Non Phospho-peptide (BP7970d) per dot were adsorbed. Antobodies working concentration was 0.5ug per ml. P-Pab: phosphorylated antibody; NP-Pab: non-phophorylated antibody; P-Pep: phospho-peptide; NP-Pep: non-phospho-peptide.
  • 14 - Phospho-TNIK(S764) Antibody AP3276A
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, DB, IHC-P, E
Primary Accession Q9UKE5
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 154943 Da
Additional Information
Gene ID 23043
Other Names TRAF2 and NCK-interacting protein kinase, TNIK, KIAA0551
Target/Specificity This TNIK Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S764 of human TNIK.
Dilution WB~~1:1000
DB~~1:500
IHC-P~~1:100~500
Format Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPhospho-TNIK(S764) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TNIK
Synonyms KIAA0551
Function Serine/threonine kinase that acts as an essential activator of the Wnt signaling pathway. Recruited to promoters of Wnt target genes and required to activate their expression. May act by phosphorylating TCF4/TCF7L2. Appears to act upstream of the JUN N- terminal pathway. May play a role in the response to environmental stress. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. More generally, it may play a role in cytoskeletal rearrangements and regulate cell spreading. Phosphorylates SMAD1 on Thr-322.
Cellular Location Nucleus. Cytoplasm. Recycling endosome. Cytoplasm, cytoskeleton. Note=Associated with recycling endosomes and the cytoskeletal fraction upon RAP2A overexpression
Tissue Location Expressed ubiquitously. Highest levels observed in heart, brain and skeletal muscle. Expressed in normal colonic epithelia and colorectal cancer tissues.
Research Areas

BACKGROUND

TNIK is a stress-activated serine/threonine kinase that may play a role in the response to environmental stress. This protein appears to act upstream of the JUN N-terminal pathway, and may play a role in cytoskeletal regulation.

REFERENCES

Taira, K., et al., J. Biol. Chem. 279(47):49488-49496 (2004).
Fu, C.A., et al., J. Biol. Chem. 274(43):30729-30737 (1999).
Yonekura, H., et al., Nucleic Acids Res. 27(13):2591-2600 (1999).

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