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Phospho-Wee1(S123) Antibody

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 6 - Phospho-Wee1(S123) Antibody AP3284a
    Dot blot analysis of anti-Phospho-Wee1-S123 Antibody (Cat. #AP3284a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antobodies working concentration was 0.5ug per ml.
  • 14 - Phospho-Wee1(S123) Antibody AP3284a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
DB, IHC-P, E
Primary Accession P30291
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 71597 Da
Additional Information
Gene ID 7465
Other Names Wee1-like protein kinase, WEE1hu, Wee1A kinase, WEE1
Target/Specificity This Wee1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S123 of human Wee1.
Dilution DB~~1:500
IHC-P~~1:100~500
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPhospho-Wee1(S123) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name WEE1
Function Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.
Cellular Location Nucleus.
Research Areas

BACKGROUND

This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase.

REFERENCES

Dai, X., et al., J. Invest. Dermatol. 122(6):1356-1364 (2004).
Watanabe, N., et al., Proc. Natl. Acad. Sci. U.S.A. 101(13):4419-4424 (2004).
Yoshida, T., et al., Ann. Oncol. 15(2):252-256 (2004).
Kawasaki, H., et al., Oncogene 22(44):6839-6844 (2003).
Yuan, H., et al., J. Virol. 77(3):2063-2070 (2003).

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