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Phospho-MYC(T58) Antibody

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 6 - Phospho-MYC(T58) Antibody AP3325a
    Dot blot analysis of Phospho-MYC-T58 Pab (Cat.AP3325a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
  • 1 - Phospho-MYC(T58) Antibody AP3325a
    Western blot analysis of Phospho-MYC-T58 Antibody in human TPA activated Hela cell line lysates. Phospho-MYC (arrow) was detected using the purified PAb. (0: without TPA; 1: 60ug/ml TPA, 15min; 2: 60ug/ml TPA, 30min; 3: 60ug/ml TPA, 45min; 4: 125ug/ml TPA, 15min; 5: 125ug/ml TPA, 30min; 6: 125ug/ml TPA, 45min; 7: 250ug/ml TPA, 15min; 8: 250ug/ml TPA, 30min; 9: 250ug/ml, 45min)
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
DB, WB, E
Primary Accession P01106
Other Accession P09416, Q29031, P01108, P01109, Q2HJ27, P24793, Q63379, P03966, P04198, Q9PSJ0, P18444, P15171, Q7ZVS9, P52160, P06171
Reactivity Human
Predicted Xenopus, Zebrafish, Chicken, Mouse, Rat, Bovine, Pig
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 48804 Da
Additional Information
Gene ID 4609
Other Names Myc proto-oncogene protein, Class E basic helix-loop-helix protein 39, bHLHe39, Proto-oncogene c-Myc, Transcription factor p64, MYC, BHLHE39
Target/Specificity This MYC Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T58 of human MYC.
Dilution DB~~1:500
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPhospho-MYC(T58) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MYC
Synonyms BHLHE39
Function Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3' (PubMed:24940000, PubMed:25956029). Activates the transcription of growth-related genes (PubMed:24940000, PubMed:25956029). Binds to the VEGFA promoter, promoting VEGFA production and subsequent sprouting angiogenesis (PubMed:24940000, PubMed:25956029). Regulator of somatic reprogramming, controls self-renewal of embryonic stem cells (By similarity). Functions with TAF6L to activate target gene expression through RNA polymerase II pause release (By similarity). Positively regulates transcription of HNRNPA1, HNRNPA2 and PTBP1 which in turn regulate splicing of pyruvate kinase PKM by binding repressively to sequences flanking PKM exon 9, inhibiting exon 9 inclusion and resulting in exon 10 inclusion and production of the PKM M2 isoform (PubMed:20010808).
Cellular Location Nucleus, nucleoplasm. Nucleus, nucleolus
Research Areas

BACKGROUND

MYC participates in the regulation of gene transcription. It binds DNA both in a non-specific manner and also specifically to recognizes the core sequence 5'-CAC[GA]TG-3'. This protein appears to activate the transcription of growth-related genes. Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors. A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia.

REFERENCES

Qi, Y., et al., Nature 431(7009):712-717 (2004).
Wilda, M., et al., Genes Chromosomes Cancer 41(2):178-182 (2004).
Dom, et al., Oncogene 23(44):7378-7390 (2004).
Pap, T., et al., Arthritis Rheum. 50(9):2794-2802 (2004).
Ozawa, N., et al., Endocrinology 145(9):4244-4250 (2004).

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