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Phospho-NFATC2(S330) Antibody

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - Phospho-NFATC2(S330) Antibody AP3429a
    Dot blot analysis of Phospho-NFATC2(S330) Antibody (Cat. AP3429a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antobodies working concentration was 0. 5ug per ml.
  • 6 - Phospho-NFATC2(S330) Antibody AP3429a
    Dot blot analysis of anti-NFATC2-pS330 Phospho-specific Pab (Cat.#AP3429a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, DB, E
Primary Accession Q13469
Other Accession NP_036472
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 100146 Da
Additional Information
Gene ID 4773
Other Names Nuclear factor of activated T-cells, cytoplasmic 2, NF-ATc2, NFATc2, NFAT pre-existing subunit, NF-ATp, T-cell transcription factor NFAT1, NFATC2, NFAT1, NFATP
Target/Specificity This NFATC2 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S330 of human NFATC2.
Dilution WB~~1:1000
DB~~1:500
E~~Use at an assay dependent concentration.
Format Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPhospho-NFATC2(S330) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NFATC2
Synonyms NFAT1, NFATP
Function Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha or GM-CSF (PubMed:15790681). Promotes invasive migration through the activation of GPC6 expression and WNT5A signaling pathway (PubMed:21871017). Is involved in the negative regulation of chondrogenesis (PubMed:35789258). Recruited by AKAP5 to ORAI1 pore- forming subunit of CRAC channels in Ca(2+) signaling microdomains where store-operated Ca(2+) influx is coupled to calmodulin and calcineurin signaling and activation of NFAT-dependent transcriptional responses.
Cellular Location Cytoplasm. Nucleus. Note=Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription
Tissue Location Expressed in thymus, spleen, heart, testis, brain, placenta, muscle and pancreas. Isoform 1 is highly expressed in the small intestine, heart, testis, prostate, thymus, placenta and thyroid Isoform 3 is highly expressed in stomach, uterus, placenta, trachea and thyroid.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

NFATC2 is a member of the nuclear factor of activated T cells (NFAT) family. It is a DNA-binding protein with a REL-homology region (RHR) and an NFAT-homology region (NHR). This protein is present in the cytosol and only translocates to the nucleus upon T cell receptor (TCR) stimulation, where it becomes a member of the nuclear factors of activated T cells transcription complex. This complex plays a central role in inducing gene transcription during the immune response.

REFERENCES

Golks,A., EMBO J. 26 (20), 4368-4379 (2007)
Dong,X., J. Biol. Chem. 282 (41), 30303-30310 (2007)
Gibson,H.M., J. Immunol. 179 (6), 3831-3840 (2007)

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