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Phospho-MeCP2(S80) Antibody

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 6 - Phospho-MeCP2(S80) Antibody AP3595a
    Dot blot analysis of anti-Phospho-MeCP2-pS80 Antibody (Cat.#AP3595a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
DB, E
Primary Accession P51608
Other Accession Q00566, Q9Z2D6, Q95LG8
Reactivity Human
Predicted Mouse, Monkey, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 52441 Da
Additional Information
Gene ID 4204
Other Names Methyl-CpG-binding protein 2, MeCp-2 protein, MeCp2, MECP2
Target/Specificity This MeCP2 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S80 of human MeCP2.
Dilution DB~~1:500
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPhospho-MeCP2(S80) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MECP2
Function Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)- containing DNA, with a preference for 5-methylcytosine (5mC).
Cellular Location Nucleus {ECO:0000250|UniProtKB:Q9Z2D6}. Note=Colocalized with methyl-CpG in the genome. Colocalized with TBL1X to the heterochromatin foci.
Tissue Location Present in all adult somatic tissues tested.
Research Areas

BACKGROUND

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of some cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females.

REFERENCES

Mnatzakanian, G.N., et al., Nat. Genet. 36(4):339-341 (2004).
Laccone, F., et al., Hum. Mutat. 23(3):234-244 (2004).
Suzuki, M., et al., Oncogene 22(54):8688-8698 (2003).
Balmer, D., et al., J. Mol. Med. 81(1):61-68 (2003).
Hagberg, B., et al., Eur. J. Paediatr. Neurol. 7(6):417-421 (2003).

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