癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
Phospho-TOPBP1(S1159) Antibody
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- 产品详情
- 文献引用 : 5
- 实验流程
- 背景知识
Application 
| DB, E |
|---|---|
| Primary Accession | Q92547 |
| Other Accession | Q6ZQF0, NP_008958.2 |
| Reactivity | Human |
| Predicted | Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 170679 Da |
| Gene ID | 11073 |
|---|---|
| Other Names | DNA topoisomerase 2-binding protein 1, DNA topoisomerase II-beta-binding protein 1, TopBP1, DNA topoisomerase II-binding protein 1, TOPBP1, KIAA0259 |
| Target/Specificity | This TOPBP1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S1159 of human TOPBP1. |
| Dilution | DB~~1:500 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Phospho-TOPBP1(S1159) Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | TOPBP1 |
|---|---|
| Synonyms | KIAA0259 |
| Function | Required for DNA replication. Plays a role in the rescue of stalled replication forks and checkpoint control (PubMed:33592542). Binds double-stranded DNA breaks and nicks as well as single-stranded DNA. Recruits the SWI/SNF chromatin remodeling complex to E2F1- responsive promoters. Down-regulates E2F1 activity and inhibits E2F1- dependent apoptosis during G1/S transition and after DNA damage. Induces a large increase in the kinase activity of ATR (PubMed:16530042). |
| Cellular Location | Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole Chromosome. Note=Detected on unpaired autosomes in meiotic prophase cells. Detected on X and Y chromosomes during later stages of prophase Colocalizes with ATR and H2AX at unsynapsed chromosome cores during prophase (By similarity). Has a uniform nuclear distribution during G phase. Colocalizes with BRCA1 at stalled replication forks during S phase. In mitotic cells it colocalizes with BRCA1 at spindle poles and centrosomes during metaphase and anaphase. Detected in discrete foci together with PML and numerous DNA repair enzymes after DNA damage by alkylating agents, UV or gamma irradiation. Localizes to sites of DNA damage in a H2AX- independent manner. |
| Tissue Location | Highly expressed in heart, brain, placenta, lung and kidney. |
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
This gene encodes a binding protein which interacts with the C-terminal region of topoisomerase II beta. This interaction suggests a supportive role for this protein in the catalytic reactions of topoisomerase II beta through transient breakages of DNA strands.
REFERENCES
Huo, Y.G., et al. Biochem. Biophys. Res. Commun. 401(3):401-405(2010)
Blackford, A.N., et al. Proc. Natl. Acad. Sci. U.S.A. 107(27):12251-12256(2010)
Takeishi, Y., et al. Genes Cells 15(7):761-771(2010)
Gong, Z., et al. Mol. Cell 37(3):438-446(2010)
Couch, F.J., et al. Cancer Epidemiol. Biomarkers Prev. 19(1):251-257(2010)
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