|Application ||WB, IHC-P, E|
|Calculated MW||119749 Da|
|Other Names||Protein transport protein Sec24A, SEC24-related protein A, SEC24A|
|Target/Specificity||This SC24A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 636-663 amino acids from the Central region of human SC24A.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||SC24A Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules for their transport to the Golgi complex (PubMed:20427317, PubMed:17499046, PubMed:18843296). Plays a central role in cargo selection within the COPII complex and together with SEC24B may have a different specificity compared to SEC24C and SEC24D. May package preferentially cargos with cytoplasmic DxE or LxxLE motifs and may also recognize conformational epitopes (PubMed:17499046, PubMed:18843296).|
|Cellular Location||Cytoplasmic vesicle, COPII-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytosol|
Provided below are standard protocols that you may find useful for product applications.
SC24A is a component of coat protein II (COPII; see MIM 601924)-coated vesicles that mediate protein transport from the endoplasmic reticulum. SEC24A is 1 of several mammalian proteins that show structural and functional homology to yeast Sec24.
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Mancias, J.D., et al. Mol. Cell 26(3):403-414(2007)
Wendeler, M.W., et al. EMBO Rep. 8(3):258-264(2007)