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RPS15A Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - RPS15A Antibody (N-term) AP4804a
    All lanes : Anti-RPS15A Antibody (N-term) at 1:1000 dilution Lane 1: A549 whole cell lysate Lane 2: K562 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 15 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - RPS15A Antibody (N-term) AP4804a
    RPS15A Antibody (N-term) (Cat. #AP4804a) IHC analysis in formalin fixed and paraffin embedded colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RPS15A Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • 4 - RPS15A Antibody (N-term) AP4804a
    RPS15A Antibody (N-term) (Cat. #AP4804a) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, FC, WB, E
Primary Accession P62244
Other Accession P62246, P62245, Q76I82
Reactivity Human
Predicted Bovine, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 14840 Da
Antigen Region 1-30 aa
Additional Information
Gene ID 6210
Other Names 40S ribosomal protein S15a, RPS15A
Target/Specificity This RPS15A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human RPS15A.
Dilution IHC-P~~1:100~500
FC~~1:10~50
WB~~1:1000
E~~Use at an assay dependent concentration.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRPS15A Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RPS15A (HGNC:10389)
Function Component of the small ribosomal subunit (PubMed:23636399). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre- ribosomal RNA by the RNA exosome (PubMed:34516797). Required for proper erythropoiesis (PubMed:27909223).
Cellular Location Cytoplasm. Nucleus, nucleolus
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

RPS15A consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S8P family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

REFERENCES

Yu, Y., et al. Protein Sci. 14(6):1438-1446(2005)
Lian, Z., et al. Mol. Carcinog. 40(1):34-46(2004)
Lehner, B., et al. Genomics 83(1):153-167(2004)

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