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Anti-FABP1 Antibody

Western blot analysis of FABP1 expression in HeLa (A), Jurkat (B), rat liver (C) whole cell lysates. (Predicted band size: 14 kD; Observed band size: 14 kD)
Immunofluorescent analysis of FABP1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	P07148
Reactivity	Human, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	14208 Da

Additional Information
Gene ID	2168
Other Names	FABPL; Fatty acid-binding protein, liver; Fatty acid-binding protein 1; Liver-type fatty acid-binding protein; L-FABP
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human FABP1. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	FABP1
Synonyms	FABPL
Function	Plays a role in lipoprotein-mediated cholesterol uptake in hepatocytes (PubMed:25732850). Binds cholesterol (PubMed:25732850). Binds free fatty acids and their coenzyme A derivatives, bilirubin, and some other small molecules in the cytoplasm. May be involved in intracellular lipid transport (By similarity).
Cellular Location	Cytoplasm.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Chan L.,et al.J. Biol. Chem. 260:2629-2632(1985).
Lowe J.B.,et al.J. Biol. Chem. 260:3413-3417(1985).
Burkard T.R.,et al.BMC Syst. Biol. 5:17-17(2011).
Xu Y.,et al.J. Am. Chem. Soc. 129:7722-7723(2007).

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¥ 1,250.00

Cat# AP50718

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Anti-FABP1 Antibody

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