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Anti-Claudin 11 Antibody

Western blot analysis of Claudin 11 expression in HeLa (A), Raw264.7 (B), mouse brain (C), rat heart (D) whole cell lysates. (Predicted band size: 21 kD; Observed band size: 21 kD)
Immunofluorescent analysis of Claudin 11 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	O75508
Reactivity	Human, Mouse, Rat, Rabbit, Chicken, Bovine, Dog
Host	Rabbit
Clonality	Polyclonal
Calculated MW	21993 Da

Additional Information
Gene ID	5010
Other Names	OSP; OTM; Claudin-11; Oligodendrocyte-specific protein
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Claudin 11. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	CLDN11
Synonyms	OSP, OTM
Function	Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.
Cellular Location	Cell junction, tight junction. Cell membrane; Multi-pass membrane protein

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Bronstein J.M.,et al.J. Neurosci. Res. 59:706-711(2000).
Keen T.J.,et al.Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases.
Kalnine N.,et al.Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.

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¥ 1,250.00

Cat# AP50727

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Anti-Claudin 11 Antibody

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