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Anti-EAAT3 Antibody

Western blot analysis of EAAT3 expression in PANC1 (A), HeLa (B), human testis (C) whole cell lysates. (Predicted band size: 57 kD; Observed band size: 57 kD)
Immunofluorescent analysis of EAAT3 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	P43005
Reactivity	Human, Mouse, Rat, Mk
Host	Rabbit
Clonality	Polyclonal
Calculated MW	57100 Da

Additional Information
Gene ID	6505
Other Names	EAAC1; EAAT3; Excitatory amino acid transporter 3; Excitatory amino-acid carrier 1; Neuronal and epithelial glutamate transporter; Sodium-dependent glutamate/aspartate transporter 3; Solute carrier family 1 member 1
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human EAAT3. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	SLC1A1 (HGNC:10939)
Function	Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate (PubMed:21123949, PubMed:26690923, PubMed:33658209, PubMed:7521911, PubMed:7914198, PubMed:8857541). Can also transport L-cysteine (PubMed:21123949). Functions as a symporter that transports one amino acid molecule together with two or three Na(+) ions and one proton, in parallel with the counter-transport of one K(+) ion (PubMed:26690923, PubMed:33658209, PubMed:7521911, PubMed:8857541). Mediates Cl(-) flux that is not coupled to amino acid transport; this avoids the accumulation of negative charges due to aspartate and Na(+) symport (PubMed:26690923, PubMed:8857541). Plays an important role in L- glutamate and L-aspartate reabsorption in renal tubuli (PubMed:21123949). Plays a redundant role in the rapid removal of released glutamate from the synaptic cleft, which is essential for terminating the postsynaptic action of glutamate (By similarity). Contributes to glutathione biosynthesis and protection against oxidative stress via its role in L-glutamate and L-cysteine transport (By similarity). Negatively regulated by ARL6IP5 (By similarity).
Cellular Location	Cell membrane; Multi-pass membrane protein {ECO:0000250\|UniProtKB:P43003}. Apical cell membrane; Multi-pass membrane protein {ECO:0000250\|UniProtKB:P43003}. Synapse, synaptosome {ECO:0000250\|UniProtKB:P51906}. Early endosome membrane {ECO:0000250\|UniProtKB:P51906}. Late endosome membrane {ECO:0000250\|UniProtKB:P51906}. Recycling endosome membrane {ECO:0000250\|UniProtKB:P51906}
Tissue Location	Expressed in all tissues tested including liver, muscle, testis, ovary, retinoblastoma cell line, neurons and brain (in which there was dense expression in substantia nigra, red nucleus, hippocampus and in cerebral cortical layers)

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Shashidharan P.,et al.Brain Res. 662:245-250(1994).
Kanai Y.,et al.J. Biol. Chem. 269:20599-20606(1994).
Arriza J.L.,et al.J. Neurosci. 14:5559-5569(1994).
Veenstra-VanderWeele J.,et al.Mol. Psychiatry 6:160-167(2001).
Yasuda-Kamatani Y.,et al.Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases.

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Cat# AP50729

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Anti-EAAT3 Antibody

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