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Anti-EAAT3 Antibody

Western blot analysis of EAAT3 expression in PANC1 (A), HeLa (B), human testis (C) whole cell lysates. (Predicted band size: 57 kD; Observed band size: 57 kD)
Immunofluorescent analysis of EAAT3 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

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实验流程

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	P43005
Reactivity	Human, Mouse, Rat, Mk
Host	Rabbit
Clonality	Polyclonal
Calculated MW	57 KDa

Additional Information
Other Names	EAAC1; EAAT3; Excitatory amino acid transporter 3; Excitatory amino-acid carrier 1; Neuronal and epithelial glutamate transporter; Sodium-dependent glutamate/aspartate transporter 3; Solute carrier family 1 member 1
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human EAAT3. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Shashidharan P.,et al.Brain Res. 662:245-250(1994).
Kanai Y.,et al.J. Biol. Chem. 269:20599-20606(1994).
Arriza J.L.,et al.J. Neurosci. 14:5559-5569(1994).
Veenstra-VanderWeele J.,et al.Mol. Psychiatry 6:160-167(2001).
Yasuda-Kamatani Y.,et al.Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases.

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¥ 1,250.00

Cat# AP50729

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Anti-EAAT3 Antibody

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