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Anti-LZTR1 Antibody

Western blot analysis of LZTR1 expression in Jurkat (A), MCF7 (B), NIHC3T3 (C), PC12 (D) whole cell lysates. (Predicted band size: 94 kD; Observed band size: 94 kD)
Immunofluorescent analysis of LZTR1 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	Q8N653
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	94719 Da

Additional Information
Gene ID	8216
Other Names	TCFL2; Leucine-zipper-like transcriptional regulator 1; LZTR-1
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human LZTR1. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	LZTR1 {ECO:0000303\|PubMed:7633402, ECO:0000312\|HGNC:HGNC:6742}
Function	Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex that mediates ubiquitination of Ras GTPases (M-Ras/MRAS, K-Ras/KRAS, N-Ras/NRAS and H-Ras/HRAS) and Ras- like protein RIT1 (PubMed:30442762, PubMed:30442766, PubMed:30481304, PubMed:40934300). Acts as a negative regulator of RAS-MAPK signaling by controlling Ras levels and decreasing Ras association with membranes (PubMed:30442762, PubMed:30442766, PubMed:30481304, PubMed:40934300).
Cellular Location	Endomembrane system. Recycling endosome. Golgi apparatus

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Collins J.E.,et al.Genome Biol. 5:R84.1-R84.11(2004).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Kurahashi H.,et al.Hum. Mol. Genet. 4:541-549(1995).
Van Damme P.,et al.Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).

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¥ 1,250.00

Cat# AP50731

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Anti-LZTR1 Antibody

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