癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
Anti-GAPDH(3E12) antibody
Purified Mouse Monoclonal Antibody (Mon)
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Application 
| WB, IHC-P, IHC-F, IF, ICC |
|---|---|
| Primary Accession | P04797 |
| Reactivity | Mouse, Rat, Rabbit |
| Host | Mouse |
| Clonality | Monoclonal |
| Calculated MW | 35828 Da |
| Gene ID | 24383 |
|---|---|
| Other Names | Glyceraldehyde-3-phosphate dehydrogenase, GAPDH, 38 kDa BFA-dependent ADP-ribosylation substrate, BARS-38, Peptidyl-cysteine S-nitrosylase GAPDH, 2699-, Gapdh, Gapd |
| Dilution | WB=1:1000-5000,IHC-P=1:100-500,IHC-F=1:100-500,ICC=1:100,IF=1:100-500 |
| Format | 0.01M TBS(pH7.4) with 1% BSA, 0.09% (W/V) sodium azide and 50% Glyce |
| Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| Name | Gapdh |
|---|---|
| Synonyms | Gapd |
| Function | Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively (PubMed:15951807, PubMed:17934141, PubMed:20972425). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D- glyceroyl phosphate (PubMed:17934141). Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (PubMed:15312048). Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop- containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation (By similarity). Also plays a role in innate immunity by promoting TNF-induced NF-kappa- B activation and type I interferon production, via interaction with TRAF2 and TRAF3, respectively (By similarity). Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis (PubMed:10424669, PubMed:15951807, PubMed:20972425). Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (PubMed:15951807, PubMed:20972425). |
| Cellular Location | Cytoplasm, cytosol. Cytoplasm, cytoskeleton. Nucleus. Note=Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (PubMed:15951807). Colocalizes with CHP1 to small punctate structures along the microtubules tracks (PubMed:15312048). |
| Tissue Location | High levels in skeletal muscle and heart, low levels in liver, brain, and kidney. |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3- phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules. Also participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S- nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation (By similarity).
REFERENCES
Fort P.,et al.Nucleic Acids Res. 13:1431-1442(1985).
Tso J.Y.,et al.Nucleic Acids Res. 13:2485-2502(1985).
Tajima H.,et al.NeuroReport 10:2029-2033(1999).
Zheng J.,et al.Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases.
Lubec G.,et al.Submitted (APR-2007) to UniProtKB.
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