Anti-ACRBP Antibody

Western blot analysis of ACRBP expression in HEK293T (A), HeLa (B) whole cell lysates. (Predicted band size: 61 kD; Observed band size: 61 kD)
Immunohistochemical analysis of ACRBP staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of ACRBP staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IF/IC
Primary Accession	Q8NEB7
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Calculated MW	61359 Da

Additional Information
Gene ID	84519
Other Names	Acrosin-binding protein; Cancer/testis antigen 23; CT23; Cancer/testis antigen OY-TES-1; Proacrosin-binding protein sp32
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ACRBP. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500 IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	ACRBP {ECO:0000312\|EMBL:AAH33010.1}
Function	[Acrosin-binding protein, mature form]: Acrosomal protein that maintains proacrosin (pro-ACR) as an enzymatically inactive zymogen in the acrosome. Involved also in the acrosome formation.
Cellular Location	Secreted {ECO:0000250\|UniProtKB:Q29016}. Cytoplasmic vesicle, secretory vesicle, acrosome {ECO:0000250\|UniProtKB:Q29016, ECO:0000250\|UniProtKB:Q3V140}
Tissue Location	Expression restricted to testis in normal tissue. Expressed in a wide spectrum of cancers, including bladder, breast, liver, lung and colon cancers.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.