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Anti-Alpha-actin-1 Antibody

Western blot analysis of Alpha-actin-1 expression in A549 (A), SP2/0 (B), rat kidney (C) whole cell lysates. (Predicted band size: 42 kD; Observed band size: 42 kD)
Immunohistochemical analysis of Alpha-actin-1 staining in human muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunoprecipitation of Alpha-actin-1 from 0.5mg Mouse Skeletal Muscle tissue lysate, using 5ug of Anti-Alpha-actin-1 Antibody and 50ul of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Mouse Skeletal Muscle tissue lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation. Proteins were eluted by addition of 40ul SDS loading buffer and incubated for 10min at 70°C; 10ul of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with Anti-Alpha-actin-1 Antibody.

产品详情
实验流程

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IP
Primary Accession	P68133
Reactivity	Human, Mouse, Rat, Rabbit, Pig, Chicken, Bovine
Host	Rabbit
Clonality	Polyclonal
Calculated MW	42051 Da

Additional Information
Gene ID	58
Other Names	ACTA; Actin alpha skeletal muscle; Alpha-actin-1
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Alpha-actin-1. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500 IP~~IP (1/10 - 1/100)
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	ACTA1
Synonyms	ACTA
Function	Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Cellular Location	Cytoplasm, cytoskeleton.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Hanauer A.,et al.Nucleic Acids Res. 11:3503-3516(1983).
Taylor A.,et al.Genomics 3:323-336(1988).
Nowak K.J.,et al.Nat. Genet. 23:208-212(1999).
Ebert L.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Gregory S.G.,et al.Nature 441:315-321(2006).

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¥ 1,250.00

Cat# AP50958

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Anti-Alpha-actin-1 Antibody

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