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Anti-ACTN3 Antibody

Western blot analysis of ACTN3 expression in HeLa (A), mouse muscle (B), rat muscle (C) whole cell lysates. (Predicted band size: 103 kD; Observed band size: 100 kD)
Immunofluorescent analysis of ACTN3 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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实验流程

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	Q08043
Reactivity	Human, Mouse, Rat, Rabbit, Bovine
Host	Rabbit
Clonality	Polyclonal
Calculated MW	103241 Da

Additional Information
Gene ID	89
Other Names	Alpha-actinin-3; Alpha-actinin skeletal muscle isoform 3; F-actin cross-linking protein
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human ACTN3. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	ACTN3
Function	F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
Tissue Location	Expression restricted to fast (type 2) skeletal muscle fibers (at protein level).

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Beggs A.H.,et al.J. Biol. Chem. 267:9281-9288(1992).
Taylor T.D.,et al.Nature 440:497-500(2006).
Mills M.,et al.Hum. Mol. Genet. 10:1335-1346(2001).
Takada F.,et al.Proc. Natl. Acad. Sci. U.S.A. 98:1595-1600(2001).
Franzot G.,et al.J. Mol. Biol. 348:151-165(2005).

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¥ 1,250.00

Cat# AP50960

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100 µl

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Anti-ACTN3 Antibody

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