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Anti-ALDOA Antibody

Western blot analysis of ALDOA expression in Hela (A), HGC27 (B), mouse spleen (C), mouse lung (D) whole cell lysates. (Predicted band size: 39 kD; Observed band size: 39 kD)
Immunofluorescent analysis of ALDOA staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	P04075
Reactivity	Human, Mouse, Rat, Rabbit, Dog
Host	Rabbit
Clonality	Polyclonal
Calculated MW	39420 Da

Additional Information
Gene ID	226
Other Names	ALDA; Fructose-bisphosphate aldolase A; Lung cancer antigen NY-LU-1; Muscle-type aldolase
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human ALDOA. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	ALDOA (HGNC:414)
Synonyms	ALDA
Function	Catalyzes the reversible conversion of beta-D-fructose 1,6- bisphosphate (FBP) into two triose phosphate and plays a key role in glycolysis and gluconeogenesis (PubMed:14766013). In addition, also functions as a scaffolding protein (By similarity). In response to glucose deprivation, FBP dissociates from aldolase and acts as an adapter that promotes AMP-activated protein kinase (AMPK) activity: mechanistically, associates with transient receptor potential channels TrpV (TRPV1-TRPV4), promoting inhibition of the V-ATPase complex on lysosomes and AMPK activation via the AXIN1-STK11/LKB1 axis (By similarity).
Cellular Location	Cytoplasm, myofibril, sarcomere, I band {ECO:0000250\|UniProtKB:P00883}. Cytoplasm, myofibril, sarcomere, M line {ECO:0000250\|UniProtKB:P00883}. Note=In skeletal muscle, accumulates around the M line and within the I band, colocalizing with FBP2 on both sides of the Z line in the absence of Ca(2+) {ECO:0000250\|UniProtKB:P00883}

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Sakakibara M.,et al.Biochem. Biophys. Res. Commun. 131:413-420(1985).
Izzo P.,et al.Eur. J. Biochem. 164:9-13(1987).
Izzo P.,et al.Eur. J. Biochem. 174:569-578(1988).
Mukai T.,et al.Eur. J. Biochem. 195:781-787(1991).
Ota T.,et al.Nat. Genet. 36:40-45(2004).

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Cat# AP50972

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Anti-ALDOA Antibody

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