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Anti-Apolipoprotein F Antibody

     
  • 1 - Anti-Apolipoprotein F Antibody AP50987
    Western blot analysis of Apolipoprotein F expression in HEK293T (A), Hela (B), mouse kidney (C), mouse liver (D), rat kidney (E), rat liver (F) whole cell lysates. (Predicted band size: 35 kD; Observed band size: 36 kD)
  • 19 - Anti-Apolipoprotein F Antibody AP50987
    Immunohistochemical analysis of Apolipoprotein F staining in human tonsillitis formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC
Primary Accession Q13790
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 35399 Da
Additional Information
Gene ID 319
Other Names Apolipoprotein F; Apo-F; Lipid transfer inhibitor protein; LTIP
Target/Specificity KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Apolipoprotein F. The exact sequence is proprietary.
Dilution WB~~WB (1/500 - 1/1000)
IHC~~1:100~500
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name APOF
Function Minor apolipoprotein that associates with LDL. Inhibits cholesteryl ester transfer protein (CETP) activity and appears to be an important regulator of cholesterol transport. Also associates to a lesser degree with VLDL, Apo-AI and Apo-AII.
Cellular Location Secreted
Tissue Location Expressed by the liver and secreted in plasma.
Research Areas

REFERENCES

Day J.R.,et al.Biochem. Biophys. Res. Commun. 203:1146-1151(1994).
Wang X.,et al.J. Biol. Chem. 274:1814-1820(1999).
Scherer S.E.,et al.Nature 440:346-351(2006).
Olofsson S.-O.,et al.Biochemistry 17:1032-1036(1978).
Halim A.,et al.Mol. Cell. Proteomics 0:0-0(2011).

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