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Anti-Procalcitonin Antibody

Western blot analysis of Procalcitonin expression in mouse spleen (A), rat spleen (B) whole cell lysates. (Predicted band size: 15; 13 kD; Observed band size: 14 kD)
Immunohistochemical analysis of Procalcitonin staining in human prostate carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of Procalcitonin staining in MDAMB231 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

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实验流程

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IF/IC
Primary Accession	P01258
Other Accession	P06881
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	15467 Da

Additional Information
Gene ID	796
Other Names	CALCA; CALC1; Calcitonin gene-related peptide 1; Alpha-type CGRP; Calcitonin gene-related peptide I; CGRP-I; CALCA; CALC1; Calcitonin
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Procalcitonin. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500 IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	CALCA (HGNC:1437)
Synonyms	CALC1
Function	[Calcitonin]: Calcitonin is a peptide hormone that causes a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones. Calcitonin function is mediated by the calcitonin receptor/CALCR and the CALCR-RAMP2 (AMYR2) receptor complex (PubMed:35324283).
Cellular Location	Secreted.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Jonas V.,et al.Proc. Natl. Acad. Sci. U.S.A. 82:1994-1998(1985).
Broad P.M.,et al.Nucleic Acids Res. 17:6999-7011(1989).
Taylor T.D.,et al.Nature 440:497-500(2006).
Nelkin B.D.,et al.Biochem. Biophys. Res. Commun. 123:648-655(1984).
Edbrooke M.R.,et al.EMBO J. 4:715-724(1985).

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Cat# AP51031

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Anti-Procalcitonin Antibody

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