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Anti-Collagen 18 alpha 1 Antibody

Western blot analysis of Collagen 18 alpha 1 expression in MCF7 (A), PC12 (B) whole cell lysates. (Predicted band size: 178 kD; Observed band size: 178 kD)
Immunohistochemical analysis of Collagen 18 alpha 1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Primary Accession	P39060
Reactivity	Human, Rat, Dog
Host	Rabbit
Clonality	Polyclonal
Calculated MW	178188 Da

Additional Information
Gene ID	80781
Other Names	Collagen alpha-1(XVIII) chain
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Collagen 18 alpha 1. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	COL18A1 (HGNC:2195)
Function	Probably plays a major role in determining the retinal structure as well as in the closure of the neural tube.
Cellular Location	Secreted, extracellular space, extracellular matrix. Secreted, extracellular space, extracellular matrix, basement membrane {ECO:0000250\|UniProtKB:P39061} [Endostatin]: Secreted. Secreted, extracellular space, extracellular matrix, basement membrane
Tissue Location	Detected in placenta (at protein level) (PubMed:32337544). Present in multiple organs with highest levels in liver, lung and kidney.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Saarela J.,et al.Matrix Biol. 16:319-328(1998).
Elamaa H.,et al.Matrix Biol. 22:427-442(2003).
Hattori M.,et al.Nature 405:311-319(2000).
Oh S.P.,et al.Genomics 19:494-499(1994).
Feng Y.,et al.Sheng Wu Gong Cheng Xue Bao 17:278-282(2001).

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¥ 1,250.00

Cat# AP51100

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Anti-Collagen 18 alpha 1 Antibody

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