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Anti-Cytochrome P450 2A13 Antibody

Western blot analysis of Cytochrome P450 2A13 expression in mouse lung (A), mouse liver (B), rat lung (C), rat liver (D) whole cell lysates. (Predicted band size: 56 kD; Observed band size: 57 kD)
Immunohistochemical analysis of Cytochrome P450 2A13 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of Cytochrome P450 2A13 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IF/IC
Primary Accession	Q16696
Reactivity	Human, Mouse, Rat, Rabbit, Dog
Host	Rabbit
Clonality	Polyclonal
Calculated MW	56688 Da

Additional Information
Gene ID	1553
Other Names	Cytochrome P450 2A13; CYPIIA13
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Cytochrome P450 2A13. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500 IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	CYP2A13
Function	Exhibits a coumarin 7-hydroxylase activity. Active in the metabolic activation of hexamethylphosphoramide, N,N-dimethylaniline, 2'-methoxyacetophenone, N-nitrosomethylphenylamine, and the tobacco- specific carcinogen, 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone. Possesses phenacetin O-deethylation activity.
Cellular Location	Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein
Tissue Location	Expressed in liver and a number of extrahepatic tissues, including nasal mucosa, lung, trachea, brain, mammary gland, prostate, testis, and uterus, but not in heart, kidney, bone marrow, colon, small intestine, spleen, stomach, thymus, or skeletal muscle

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Fernandez-Salguero P.,et al.Am. J. Hum. Genet. 57:651-660(1995).
Su T.,et al.Cancer Res. 60:5074-5079(2000).
Cauffiez C.,et al.Biochem. Biophys. Res. Commun. 317:662-669(2004).
DeVore N.M.,et al.Drug Metab. Dispos. 36:2582-2590(2008).
Sansen S.,et al.Arch. Biochem. Biophys. 464:197-206(2007).

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¥ 1,250.00

Cat# AP51144

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Anti-Cytochrome P450 2A13 Antibody

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