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Anti-Cytochrome P450 2U1 Antibody

Western blot analysis of Cytochrome P450 2U1 expression in HEK293T (A), K562 (B) whole cell lysates. (Predicted band size: 61 kD; Observed band size: 62 kD)
Immunohistochemical analysis of Cytochrome P450 2U1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of Cytochrome P450 2U1 staining in LOVO cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IF/IC
Primary Accession	Q7Z449
Reactivity	Human, Mk
Host	Rabbit
Clonality	Polyclonal
Calculated MW	61987 Da

Additional Information
Gene ID	113612
Other Names	Cytochrome P450 2U1
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Cytochrome P450 2U1. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500 IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	CYP2U1 {ECO:0000303\|PubMed:14660610, ECO:0000312\|HGNC:HGNC:20582}
Function	A cytochrome P450 monooxygenase involved in the metabolism of arachidonic acid and its conjugates (PubMed:14660610, PubMed:24563460). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH- ferrihemoprotein reductase) (PubMed:14660610, PubMed:24563460). Acts as an omega and omega-1 hydroxylase for arachidonic acid and possibly for other long chain fatty acids. May modulate the arachidonic acid signaling pathway and play a role in other fatty acid signaling processes (PubMed:14660610, PubMed:24563460). May down-regulate the biological activities of N-arachidonoyl-serotonin, an endocannabinoid that has anti-nociceptive effects through inhibition of fatty acid amide hydrolase FAAH, TRPV1 receptor and T-type calcium channels. Catalyzes C-2 oxidation of the indole ring of N-arachidonoyl-serotonin forming a less active product 2-oxo-N-arachidonoyl-serotonin (PubMed:24563460).
Cellular Location	Endoplasmic reticulum membrane; Multi-pass membrane protein. Microsome membrane; Multi- pass membrane protein. Mitochondrion inner membrane; Multi-pass membrane protein
Tissue Location	Widely expressed with stronger expression in thymus, heart and cerebellum.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Chuang S.S.,et al.J. Biol. Chem. 279:6305-6314(2004).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Karlgren M.,et al.Biochem. Biophys. Res. Commun. 315:679-685(2004).
Choudhary D.,et al.Arch. Biochem. Biophys. 436:50-61(2005).
Tesson C.,et al.Am. J. Hum. Genet. 91:1051-1064(2012).

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Cat# AP51148

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Anti-Cytochrome P450 2U1 Antibody

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