癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
EIF5B Antibody
Purified Rabbit Polyclonal Antibody (Pab)
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Application 
| WB, IP, IHC-P |
|---|---|
| Primary Accession | O60841 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 138827 Da |
| Gene ID | 9669 |
|---|---|
| Other Names | Eukaryotic translation initiation factor 5B, eIF-5B, Translation initiation factor IF-2, EIF5B, IF2, KIAA0741 |
| Dilution | WB~~1:1000 IP~~N/A IHC-P~~1:50~200 |
| Format | 0.01M PBS, pH 7.2, 0.09% (W/V) Sodium azide, Glycerol 50% |
| Storage | Store at -20 °C.Stable for 12 months from date of receipt |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | EIF5B |
|---|---|
| Synonyms | IF2, KIAA0741 |
| Function | Plays a role in translation initiation (PubMed:10659855, PubMed:35732735). Ribosome-dependent GTPase that promotes the joining of the 60S ribosomal subunit to the pre-initiation complex to form the 80S initiation complex with the initiator methionine-tRNA in the P-site base paired to the start codon (PubMed:10659855, PubMed:35732735). Together with eIF1A (EIF1AX), actively orients the initiator methionine-tRNA in a conformation that allows 60S ribosomal subunit joining to form the 80S initiation complex (PubMed:12569173, PubMed:35732735). Is released after formation of the 80S initiation complex (PubMed:35732735). Its GTPase activity is not essential for ribosomal subunits joining, but GTP hydrolysis is needed for eIF1A (EIF1AX) ejection quickly followed by EIF5B release to form elongation- competent ribosomes (PubMed:10659855, PubMed:35732735). In contrast to its procaryotic homolog, does not promote recruitment of Met-rRNA to the small ribosomal subunit (PubMed:10659855). |
| Cellular Location | Cytoplasm {ECO:0000250|UniProtKB:Q05D44}. |
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Function in general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes. Seems to function along with eIF-2 (By similarity).
REFERENCES
Wilson S.A.,et al.Biochem. J. 342:97-103(1999).
Lee J.H.,et al.Proc. Natl. Acad. Sci. U.S.A. 96:4342-4347(1999).
Nagase T.,et al.DNA Res. 5:277-286(1998).
Hillier L.W.,et al.Nature 434:724-731(2005).
Bienvenut W.V.,et al.Submitted (MAR-2009) to UniProtKB.
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