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>   首页   >   产品   >   一抗   >   癌症   >   ADFP Antibody (Center)   

ADFP Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - ADFP Antibody (Center) AP5118c
    All lanes: Anti-ADFP Antibody (Center) at 1:1000 dilution Lane 1: HepG2 whole cell lysate Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 µg per lane. Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ASP1615) at 1/15000 dilution. Observed band size: 48 KDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - ADFP Antibody (Center) AP5118c
    ADFP Antibody (Center) (Cat. #AP5118c) IHC analysis in formalin fixed and paraffin embedded breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ADFP Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
  • 4 - ADFP Antibody (Center) AP5118c
    ADFP Antibody (Center) (Cat. #AP5118c) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, FC, E
Primary Accession Q99541
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 48075 Da
Antigen Region 186-214 aa
Additional Information
Gene ID 123
Other Names Perilipin-2, Adipophilin, Adipose differentiation-related protein, ADRP, PLIN2, ADFP
Target/Specificity This ADFP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 186-214 amino acids from the Central region of human ADFP.
Dilution WB~~1:1000
IHC-P~~1:100~500
FC~~1:10~50
E~~Use at an assay dependent concentration.
Format Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsADFP Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PLIN2 (HGNC:248)
Synonyms ADFP
Function Structural component of lipid droplets, which is required for the formation and maintenance of lipid storage droplets.
Cellular Location Membrane {ECO:0000250|UniProtKB:P43883}; Peripheral membrane protein {ECO:0000250|UniProtKB:P43883}. Lipid droplet
Tissue Location Milk lipid globules..
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

Adipocyte differentiation-related protein is associated with the globule surface membrane material. This protein is a major constituent of the globule surface. Increase in mRNA levels is one of the earliest indications of adipocyte differentiation.

REFERENCES

Kimmel, A.R., et al. J. Lipid Res. 51(3):468-471(2010)
Kotokorpi, P., et al. Mol. Pharmacol. 77(1):79-86(2010)
Minnaard, R., et al. J. Clin. Endocrinol. Metab. 94(10):4077-4085(2009)

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