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Anti-EPS8L3 Antibody

Western blot analysis of EPS8L3 expression in U251MG (A) whole cell lysates. (Predicted band size: 66 kD; Observed band size: 67 kD)
Immunohistochemical analysis of EPS8L3 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of EPS8L3 staining in U251MG cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IF/IC
Primary Accession	Q8TE67
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Calculated MW	66861 Da

Additional Information
Gene ID	79574
Other Names	EPS8R3; Epidermal growth factor receptor kinase substrate 8-like protein 3; EPS8-like protein 3; Epidermal growth factor receptor pathway substrate 8-related protein 3; EPS8-related protein 3
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human EPS8L3. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500 IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	EPS8L3
Synonyms	EPS8R3
Cellular Location	Cytoplasm.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Tocchetti A.,et al.Genomics 81:234-244(2003).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Gregory S.G.,et al.Nature 441:315-321(2006).
Offenhaeuser N.,et al.Mol. Biol. Cell 15:91-98(2004).
Voss M.,et al.BMC Immunol. 10:53-53(2009).

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¥ 1,250.00

Cat# AP51191

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Anti-EPS8L3 Antibody

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