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Anti-ETS1 (Phospho-T38) Antibody

Western blot analysis of ETS1 (Phospho-T38) expression in LO2 (A), Jurkat (B), HepG2 (C), NIHC3T3 (D), rat liver (E) whole cell lysates. (Predicted band size: 50 kD; Observed band size: 54 kD)
Immunohistochemical analysis of ETS1 (Phospho-T38) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of ETS1 (Phospho-T38) staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IF/IC
Primary Accession	P14921
Reactivity	Human, Mouse, Rat, Rabbit, Pig, Chicken, Bovine, Dog
Host	Rabbit
Clonality	Polyclonal
Calculated MW	50408 Da

Additional Information
Gene ID	2113
Other Names	EWSR2; Protein C-ets-1; p54
Target/Specificity	KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T38 of human ETS1 protein. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500 IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	ETS1
Synonyms	EWSR2
Function	Transcription factor (PubMed:10698492, PubMed:11909962). Directly controls the expression of cytokine and chemokine genes in a wide variety of different cellular contexts (PubMed:20378371). May control the differentiation, survival and proliferation of lymphoid cells (PubMed:20378371). May also regulate angiogenesis through regulation of expression of genes controlling endothelial cell migration and invasion (PubMed:15247905, PubMed:15592518).
Cellular Location	Nucleus. Cytoplasm Note=Delocalizes from nucleus to cytoplasm when coexpressed with isoform Ets-1 p27.
Tissue Location	Highly expressed within lymphoid cells. Isoforms c- ETS-1A and Ets-1 p27 are both detected in all fetal tissues tested, but vary with tissue type in adult tissues. None is detected in brain or kidney.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Reddy E.S.P.,et al.Oncogene Res. 3:239-246(1988).
Watson D.K.,et al.Proc. Natl. Acad. Sci. U.S.A. 85:7862-7866(1988).
Laitem C.,et al.Oncogene 28:2087-2099(2009).
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Bechtel S.,et al.BMC Genomics 8:399-399(2007).

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¥ 1,250.00

Cat# AP51196

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Anti-ETS1 (Phospho-T38) Antibody

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