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Anti-FLI1 Antibody

Western blot analysis of FLI1 expression in THP1 (A) whole cell lysates. (Predicted band size: 50 kD; Observed band size: 51 kD)
Immunohistochemical analysis of FLI1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Primary Accession	Q01543
Reactivity	Human, Mouse, Rat, Chicken, Bovine
Host	Rabbit
Clonality	Polyclonal
Calculated MW	50982 Da

Additional Information
Gene ID	2313
Other Names	Friend leukemia integration 1 transcription factor; Proto-oncogene Fli-1; Transcription factor ERGB
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human FLI1. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	FLI1
Function	Sequence-specific transcriptional activator (PubMed:24100448, PubMed:26316623, PubMed:28255014). Recognizes the DNA sequence 5'- C[CA]GGAAGT-3'.
Cellular Location	Nucleus.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Delattre O.,et al.Nature 359:162-165(1992).
Watson D.K.,et al.Cell Growth Differ. 3:705-713(1992).
Watson D.,et al.Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases.
Prasad D.D.,et al.Cancer Res. 52:5833-5837(1992).
Hromas R.,et al.Biochim. Biophys. Acta 1172:155-158(1993).

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¥ 1,250.00

Cat# AP51209

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100 µl

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¥ 2,050.00

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Anti-FLI1 Antibody

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